منابع مشابه
Irreversible heat inactivation of DNase I without RNA degradation.
In many applications of RT-PCR, residual genomic DNA in total RNA preparations is amplified together with RNA, which results in false-positive data. One way to overcome this problem is to design primers that span a region containing one or more introns, thereby creating a diagnostic size difference between the amplification products originating from the RNA or the DNA. However, this method will...
متن کاملDNaseR: DNase I footprinting analysis of DNase-seq data
The combination of DNase I digestion and high-throughput sequencing (DNaseseq) has been used recently to map chromatin accessibility in a given tissue or cell type on a genome-wide scale (Song and Crawford, 2010). In addition to DNase I hypersensitive sites (DHSs), short regions of protected nucleotides known as footprints can be detected using a technique known as ”digital genomic footprinting...
متن کاملSynaptonemal complexes from DNase-treated rat pachytene chromosomes contain (GT)n and LINE/SINE sequences.
Purified chromosome cores (synaptonemal complexes) of rat pachytene chromosomes, from which the chromatin is removed by extensive DNase II digestion, retain a residual class of DNA, presumably the bases of chromatin loops. This synaptonemal complex-associated DNA, isolated by proteinase digestion and phenol extraction of purified DNase-treated synaptonemal complexes, and cloned in plasmid vecto...
متن کاملDNase I levels and disease outcome in JIA patients treated with etanercept
Methods The study was performed in 25 JIA patients who donated paired serum samples prior and one year after continous etanercept therapy. Basic clinical data (six core set variables defined in ACR PEDI outcome score) were recorded along with alkalyne DNase I serum levels using the method where acid soluble nucleotides are determined spectrophotometrically at 260 nm. Treatment schedule of etane...
متن کاملDNase I digestion of chromatin from avian liver nuclei liberates DNA-dependent RNA polymerase II.
In an effort to develop mild conditions for the isolation of DNA-dependent RNA polymerase II, we have used DNase I covalently coupled to Sepharose 4B to digest chromatin from hypotonically lysed nuclei from rooster liver. The RNA polymerase II released was at least 2 times more active in in vitro transcription than was RNA polymerase II prepared by the conventional method of sonication of chrom...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2012
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113836